MSCV.IRES.hCD4
(Plasmid #35712)

• Depositing Lab: Patricia Ernst
• Publication: Artinger et al Proc Natl Acad Sci U S A. 2013 Jul 16;110(29):12000-5. doi: 10.1073/pnas.1301278110. Epub 2013 Jun 6.

Backbone

• Vector backbone: MSCV 2.2
• Backbone size w/o insert (bp): 6505
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: IRES
• Insert Size (bp): 595

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcorI (unknown if destroyed)
• 3′ cloning site: EcorI (unknown if destroyed)
• 5′ sequencing primer: TGG CTC TCC TCA AGC GTA TT
• 3′ sequencing primer: CCT CAC ATT GCC AAA AGA CG

Gene/Insert 2

• Gene/Insert name: hCD4
• Species: H. sapiens (human)
• Insert Size (bp): 1381

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: None (unknown if destroyed)
• 3′ cloning site: None (unknown if destroyed)
• 5′ sequencing primer: GCG AAA ACA GGA AAG TTG CAT C

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Thomas Graf
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that the original plasmid was described in Xie et al., 2004 Cell (PubMed ID - 15163413).