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pcDNA4.1/HisMax-Syx1
(Plasmid #35708)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35708 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4/HisMax
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5250
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Syx1
  • Alt name
    synectin-binding RhoA ex-change factor
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3219
  • Mutation
    R205K
  • Entrez Gene
    Plekhg5 (a.k.a. Syx)
  • Promoter CMV
  • Tags / Fusion Proteins
    • 6x HIS (N terminal on backbone)
    • Xpress (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer CMV fwd
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This construct contains a R205K mutation when compared to GenBank ID NP_001004156.1 This mutation reflects the sequence of the original mouse cDNA clone and was not introduced erroneously. The plasmid is exactly as described in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA4.1/HisMax-Syx1 was a gift from Arie Horowitz (Addgene plasmid # 35708 ; http://n2t.net/addgene:35708 ; RRID:Addgene_35708)

  • For your References section:

    A PDZ-binding motif as a critical determinant of Rho guanine exchange factor function and cell phenotype. Liu M, Horowitz A. Mol Biol Cell. 2006 Apr;17(4):1880-7. Epub 2006 Feb 8. 10.1091/mbc.e06-01-0002 PubMed 16467373
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