pcDNA3.1-mRFP-Syx1
(Plasmid #35707)

• Depositing Lab: Arie Horowitz
• Publication: Liu et al Mol Biol Cell. 2006 Apr;17(4):1880-7. Epub 2006 Feb 8.

Backbone

• Vector backbone: pcDNA3.1-mRFP
• Backbone size w/o insert (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Syx1
• Alt name: synectin-binding RhoA ex-change factor
• Species: M. musculus (mouse)
• Insert Size (bp): 3219
• Mutation: R205K
• Entrez Gene: Plekhg5 (a.k.a. Syx)
• Promoter: CMV
• Tag / Fusion Protein:
  • mRFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NA (unknown if destroyed)
• 3′ cloning site: NA (unknown if destroyed)
• 5′ sequencing primer: RFP-F

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This construct contains a R205K mutation when compared to GenBank ID NP_001004156.1 This mutation reflects the sequence of the original mouse cDNA clone and was not introduced erroneously. The plasmid is exactly as described in the associated publication.