pEGFP-Syx1
(Plasmid
#35706)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 35706 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSyx1
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Alt namesynectin-binding RhoA ex-change factor
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SpeciesM. musculus (mouse)
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Insert Size (bp)3219
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MutationR205K
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Entrez GenePlekhg5 (a.k.a. Syx)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer EGFP-C (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This construct contains a R205K mutation when compared to GenBank ID NP_001004156.1 This mutation reflects the sequence of the original mouse cDNA clone and was not introduced erroneously. The plasmid is exactly as described in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFP-Syx1 was a gift from Arie Horowitz (Addgene plasmid # 35706 ; http://n2t.net/addgene:35706 ; RRID:Addgene_35706) -
For your References section:
A PDZ-binding motif as a critical determinant of Rho guanine exchange factor function and cell phenotype. Liu M, Horowitz A. Mol Biol Cell. 2006 Apr;17(4):1880-7. Epub 2006 Feb 8. 10.1091/mbc.e06-01-0002 PubMed 16467373