Skip to main content
Addgene

PL06010B1H03 (smedwi1)
(Plasmid #35699)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35699 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBluescript II SK(+)
  • Backbone size w/o insert (bp) 3000

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Smedwi1
  • Alt name
    Piwi1
  • Species
    S. mediterranea
  • Promoter T7 sense, T3 antisense

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer M13-20
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PL06010B1H03 (smedwi1) was a gift from Phillip Newmark (Addgene plasmid # 35699 ; http://n2t.net/addgene:35699 ; RRID:Addgene_35699)

  • For your References section:

    The planarian Schmidtea mediterranea as a model for epigenetic germ cell specification: analysis of ESTs from the hermaphroditic strain. Zayas RM, Hernandez A, Habermann B, Wang Y, Stary JM, Newmark PA. Proc Natl Acad Sci U S A. 2005 Dec 20;102(51):18491-6. Epub 2005 Dec 12. 10.1073/pnas.0509507102 PubMed 16344473
Related items: