PL06008A2C06 (smedwi2)
(Plasmid
#35698)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 35698 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript II SK(+)
- Backbone size w/o insert (bp) 3000
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSmedwi2
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Alt namePiwi2
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SpeciesS. mediterranea
- Promoter T7 sense, T3 antisense
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer M13-20
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PL06008A2C06 (smedwi2) was a gift from Phillip Newmark (Addgene plasmid # 35698 ; http://n2t.net/addgene:35698 ; RRID:Addgene_35698) -
For your References section:
The planarian Schmidtea mediterranea as a model for epigenetic germ cell specification: analysis of ESTs from the hermaphroditic strain. Zayas RM, Hernandez A, Habermann B, Wang Y, Stary JM, Newmark PA. Proc Natl Acad Sci U S A. 2005 Dec 20;102(51):18491-6. Epub 2005 Dec 12. 10.1073/pnas.0509507102 PubMed 16344473