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Addgene

pJC53.2/Smed001246
(Plasmid #35693)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35693 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJC53.2
  • Backbone manufacturer
    Jim Collins, Newmark lab

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Kanamycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Protein arginine methyltransferase 1 homolog
  • Alt name
    Smed001246
  • Species
    S. mediterranea
  • Insert Size (bp)
    800
  • GenBank ID
    JQ035531
  • Promoter Sp6 for sense, T7 for sense and antisense

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There are several mismatches between Addgene's quality control sequence and the available NCBI sequence. Depositing lab believes these mismatches will not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJC53.2/Smed001246 was a gift from Phillip Newmark (Addgene plasmid # 35693 ; http://n2t.net/addgene:35693 ; RRID:Addgene_35693)

  • For your References section:

    PRMT5 and the role of symmetrical dimethylarginine in chromatoid bodies of planarian stem cells. Rouhana L, Vieira AP, Roberts-Galbraith RH, Newmark PA. Development. 2012 Mar;139(6):1083-94. Epub 2012 Feb 8. 10.1242/dev.076182 PubMed 22318224
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