pJC53.2/PRMT5
(Plasmid
#35692)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 35692 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepJC53.2
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Backbone manufacturerJim Collins, Newmark lab
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameProtein arginine methyltransferase 5
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Alt namePRMT5
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SpeciesS. mediterranea
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Insert Size (bp)1500
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GenBank ID
- Promoter Sp6 for sense, T7 for sense and antisense
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer Sp6
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
There are several mismatches between Addgene's quality control sequence and the best match available NCBI sequence (AEX33822.1). Depositing lab believes these mismatches will not affect plasmid function.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJC53.2/PRMT5 was a gift from Phillip Newmark (Addgene plasmid # 35692 ; http://n2t.net/addgene:35692 ; RRID:Addgene_35692) -
For your References section:
PRMT5 and the role of symmetrical dimethylarginine in chromatoid bodies of planarian stem cells. Rouhana L, Vieira AP, Roberts-Galbraith RH, Newmark PA. Development. 2012 Mar;139(6):1083-94. Epub 2012 Feb 8. 10.1242/dev.076182 PubMed 22318224
