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Addgene

pCMV-GFP-MHC-IIB
(Plasmid #35691)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35691 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MHC IIB
  • Alt name
    myosin-IIB heavy chain
  • Alt name
    NMHC IIB
  • Species
    H. sapiens (human)
  • Entrez Gene
    MYH10 (a.k.a. NMMHC-IIB, NMMHCB)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site none (unknown if destroyed)
  • 3′ cloning site none (not destroyed)
  • 5′ sequencing primer EGFP-C
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-GFP-MHC-IIB was a gift from Venkaiah Betapudi (Addgene plasmid # 35691 ; http://n2t.net/addgene:35691 ; RRID:Addgene_35691)

  • For your References section:

    Myosin II motor proteins with different functions determine the fate of lamellipodia extension during cell spreading. Betapudi V. PLoS One. 2010 Jan 5;5(1):e8560. 10.1371/journal.pone.0008560 PubMed 20052411
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