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pCMV-eGFP-NMHC-IIAdeltaIQ2
(Plasmid #35690)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35690 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP
  • Backbone manufacturer
    Clontech
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MHC IIA
  • Alt name
    myosin-IIA heavy chain
  • Alt name
    NMHC IIA
  • Species
    H. sapiens (human)
  • Mutation
    The coding segment for aa 804-834 (corresponding to the IQ2 motif that binds RLC) was deleted with the deletion junction marked by an engineered NheI restriction site.
  • Entrez Gene
    MYH9 (a.k.a. BDPLT6, DFNA17, EPSTS, FTNS, MATINS, MHA, NMHC-II-A, NMMHC-IIA, NMMHCA)
  • Promoter CMV
  • Tags / Fusion Proteins
    • GFPGenotRW
    • EGFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV fwd
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-eGFP-NMHC-IIAdeltaIQ2 was a gift from Tom Egelhoff (Addgene plasmid # 35690 ; http://n2t.net/addgene:35690 ; RRID:Addgene_35690)

  • For your References section:

    Multiple regulatory steps control mammalian nonmuscle myosin II assembly in live cells. Breckenridge MT, Dulyaninova NG, Egelhoff TT. Mol Biol Cell. 2009 Jan;20(1):338-47. Epub 2008 Oct 29. 10.1091/mbc.e08-04-0372 PubMed 18971378
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