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Addgene

Lox-Stop-Lox TOPO Hygro
(Plasmid #35688)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35688 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCR-Blunt II-TOPO
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 3500
  • Vector type
    Mammalian Expression, Cre/Lox
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Plasmid yield and stability is improved when grown at 30 degrees.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    loxP-STOP-loxP
  • Alt name
    loxP STOP loxP
  • Species
    SV40
  • Insert Size (bp)
    6100

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

If you are using the cassette for gene targeting, you should check the number of SV40 repeats in the targeted locus as the lab has noticed that in some ES clones copies of the repeats were missing.

Please also see Lox-Stop-Lox TOPO (http://www.addgene.org/11584/) for a puromycin version of this plasmid and to download a document about using the Lox-Stop-Lox system.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lox-Stop-Lox TOPO Hygro was a gift from Jacqueline Lees (Addgene plasmid # 35688 ; http://n2t.net/addgene:35688 ; RRID:Addgene_35688)
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