pCMV-mCherry-MHC-IIA
(Plasmid #35687)

• Depositing Lab: Venkaiah Betapudi
• Publication: Dulyaninova et al Mol Biol Cell. 2007 Aug;18(8):3144-55. Epub 2007 Jun 13.

Backbone

• Vector backbone: pEGFP
• Backbone manufacturer: Clontech
• Modifications to backbone: EGFP replaced with mCherry [cloned from pRSET-Bm (Shaner et al., 2004)] between AgeI and HindIII.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MHC IIA
• Alt name: myosin-IIA heavy chain
• Alt name: NMHC IIA
• Species: H. sapiens (human)
• Entrez Gene: MYH9 (a.k.a. BDPLT6, DFNA17, EPSTS, FTNS, MATINS, MHA, NMHC-II-A, NMMHC-IIA, NMMHCA)
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: mCherry-F

Resource Information

• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry