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Addgene

pTWEEN-miR-224
(Plasmid #35683)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35683 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRRL-CMV-PGK-GFP-WPRE (TWEEN)
  • Backbone manufacturer
    provided by PESCHLE C. (PMID:163300772) - CONDORELLI G (PMID: 12692591)
  • Backbone size w/o insert (bp) 8600
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    nsa-mir-224
  • Alt name
    MIR224
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    561
  • GenBank ID
    407009 NC_000023.10
  • Entrez Gene
    MIR224 (a.k.a. MIRN224, miRNA224)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer GFP-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that though there is GFP in this plasmid, it is not fused to the miR-224. There is a PGK promoter between the miR and the GFP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTWEEN-miR-224 was a gift from George Calin (Addgene plasmid # 35683 ; http://n2t.net/addgene:35683 ; RRID:Addgene_35683)
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