pEGFP-MRLC T18A,S19A
(Plasmid #35681)

• Depositing Lab: Tom Egelhoff
• Publication: Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

Backbone

• Vector backbone: pEGFP
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MRLC1
• Alt name: MYL9
• Species: H. sapiens (human)
• Mutation: T18A and S19A
• Entrez Gene: MYL9 (a.k.a. LC20, MLC-2C, MLC2, MMIHS4, MRLC1, MYRL2)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: CMV fwd
• 3′ sequencing primer: EGFP-N

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is identical to the one published in Beach et al., 2011, except the tet-inducible promoter has been replaced with a constitutive CMV promoter.