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Addgene

pEGFP-N1-Pcdh19-GFP
(Plasmid #35675)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35675 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4733
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pcdh19
  • Species
    D. rerio (zebrafish)
  • Entrez Gene
    pcdh19 (a.k.a. fc83e05, wu:fc83e05)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV fwd
  • 3′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-N1-Pcdh19-GFP was a gift from James Jontes (Addgene plasmid # 35675 ; http://n2t.net/addgene:35675 ; RRID:Addgene_35675)

  • For your References section:

    Protocadherin-19 and N-cadherin interact to control cell movements during anterior neurulation. Biswas S, Emond MR, Jontes JD. J Cell Biol. 2010 Nov 29;191(5):1029-41. 10.1083/jcb.201007008 PubMed 21115806
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