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Addgene

pAAV CB6 FFLuc-miR122
(Plasmid #35656)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35656 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENN AAV CB6 FFLuc
  • Backbone size w/o insert (bp) 6051
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    pri-miR-122
  • Insert Size (bp)
    283
  • Entrez Gene
    Mir122 (a.k.a. Mir122a, Mir122b, Mirn122a, Mirn122b, mir-122, mmu-mir-122)
  • Promoter CB

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Apa I (not destroyed)
  • 3′ cloning site Apa I (not destroyed)
  • 5′ sequencing primer gcacggaaagacgatga
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV CB6 FFLuc-miR122 was a gift from Phillip Zamore (Addgene plasmid # 35656 ; http://n2t.net/addgene:35656 ; RRID:Addgene_35656)

  • For your References section:

    Long-term, efficient inhibition of microRNA function in mice using rAAV vectors. Xie J, Ameres SL, Friedline R, Hung JH, Zhang Y, Xie Q, Zhong L, Su Q, He R, Li M, Li H, Mu X, Zhang H, Broderick JA, Kim JK, Weng Z, Flotte TR, Zamore PD, Gao G. Nat Methods. 2012 Mar 4;9(4):403-9. doi: 10.1038/nmeth.1903. 10.1038/nmeth.1903 PubMed 22388288
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