pAAV CB6 FFLuc-miR122
(Plasmid #35656)

• Depositing Lab: Phillip Zamore
• Publication: Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi: 10.1038/nmeth.1903.

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Backbone

• Vector backbone: pENN AAV CB6 FFLuc
• Backbone size w/o insert (bp): 6051
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pri-miR-122
• Insert Size (bp): 283
• Entrez Gene: Mir122 (a.k.a. Mir122a, Mir122b, Mirn122a, Mirn122b, mir-122, mmu-mir-122)
• Promoter: CB

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Apa I (not destroyed)
• 3′ cloning site: Apa I (not destroyed)
• 5′ sequencing primer: gcacggaaagacgatga

Resource Information

• Supplemental Documents:
  • VNTI map of pAAV CB6 FFLuc-miR122

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry