FoxO1-ADA-GFP
(Plasmid #35640)

• Depositing Lab: Domenico Accili
• Publication: Qiang et al J Biol Chem. 2010 Aug 27;285(35):27396-401. Epub 2010 Jun 2.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Modifications to backbone: Delete MCS sequence between Nhe I and Kpn I. These two restriction sites are also destroyed.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FoxO1-ADA-GFP
• Alt name: Foxo1
• Alt name: Fkhr
• Alt name: forkhead box O1
• Species: M. musculus (mouse)
• Insert Size (bp): 1700
• Mutation: T24A, S253D and S316A mutations
• Entrez Gene: Foxo1 (a.k.a. Afxh, FKHR, Fkhr1, Foxo1a)
• Promoter: CMV
• Tags / Fusion Proteins:
  • Myc (N terminal on backbone)
  • Flag (N terminal on insert)
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn I (destroyed during cloning)
• 3′ cloning site: BamH I (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-N; SV40pA-R

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

E15G and G282R mutations are present in this insert but are not known to affect function. There are also K219R and L619P polymorphisms. Plasmid should still function as expected of the ADA.