pLenti-PGK-ER-KRAS(G12V)
(Plasmid #35635)

• Depositing Lab: Daniel Haber
• Publication: Singh et al Cell. 2012 Feb 17;148(4):639-50.

Backbone

• Vector backbone: pLenti-PGK-Hygro
• Backbone manufacturer: Eric Campeau
• Backbone size w/o insert (bp): 10000
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: K-Ras4B(G12V)
• Alt name: KRAS4B
• Species: H. sapiens (human)
• Insert Size (bp): 1100
• Mutation: Glycine 12 to Valine
• Entrez Gene: KRAS (a.k.a. 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2)
• Promoter: PGK
• Tag / Fusion Protein:
  • ER (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: attL1
• 3′ sequencing primer: attL2

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry