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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 35632 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLenti-PGK-Hygro
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Backbone manufacturerEric Campeau
- Backbone size w/o insert (bp) 10000
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Vector typeMammalian Expression, Lentiviral
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameN-Ras(G12V)
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Alt nameNRAS
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SpeciesH. sapiens (human)
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Insert Size (bp)550
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MutationGlycine 12 to Valine
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Entrez GeneNRAS (a.k.a. ALPS4, CMNS, KRAS, N-ras, NCMS, NRAS1, NS6)
- Promoter PGK
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer attL1
- 3′ sequencing primer attL2 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti-PGK-NRAS(G12V) was a gift from Daniel Haber (Addgene plasmid # 35632 ; http://n2t.net/addgene:35632 ; RRID:Addgene_35632) -
For your References section:
TAK1 Inhibition Promotes Apoptosis in KRAS-Dependent Colon Cancers. Singh A, Sweeney MF, Yu M, Burger A, Greninger P, Benes C, Haber DA, Settleman J. Cell. 2012 Feb 17;148(4):639-50. 10.1016/j.cell.2011.12.033 PubMed 22341439