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Addgene

pLenti-PGK-NRAS(G12V)
(Plasmid #35632)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35632 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pLenti-PGK-Hygro
  • Backbone manufacturer
    Eric Campeau
  • Backbone size w/o insert (bp) 10000
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    N-Ras(G12V)
  • Alt name
    NRAS
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    550
  • Mutation
    Glycine 12 to Valine
  • Entrez Gene
    NRAS (a.k.a. ALPS4, CMNS, KRAS, N-ras, NCMS, NRAS1, NS6)
  • Promoter PGK
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-PGK-NRAS(G12V) was a gift from Daniel Haber (Addgene plasmid # 35632 ; http://n2t.net/addgene:35632 ; RRID:Addgene_35632)
  • For your References section:

    TAK1 Inhibition Promotes Apoptosis in KRAS-Dependent Colon Cancers. Singh A, Sweeney MF, Yu M, Burger A, Greninger P, Benes C, Haber DA, Settleman J. Cell. 2012 Feb 17;148(4):639-50. 10.1016/j.cell.2011.12.033 PubMed 22341439