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Addgene

pmSTpCR21 Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb ladder and a digestion of the plasmid 35628 with the following enzymes: NcoI-HF single digest and NcoI-HF and XbaI double digest. We expect NcoI to give 2 bands, 4267bp and 1570bp. XbaI should cut 60bp off the smaller fragment.

We see the expected results.