pmSTpCR21
(Plasmid
#35628)
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PurposeBacterial expression of mSERT
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 35628 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCR2.1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSERT
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Alt nameSLC6A4
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Alt nameserotonin transporter
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SpeciesM. musculus (mouse)
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Entrez GeneSlc6a4 (a.k.a. 5-HTT, Htt, Sert)
- Promoter T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site none (unknown if destroyed)
- 3′ cloning site none (unknown if destroyed)
- 5′ sequencing primer T7 fwd
- 3′ sequencing primer M13-Rev (Common Sequencing Primers)
Resource Information
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Addgene Notes
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmSTpCR21 was a gift from Howard Gu (Addgene plasmid # 35628 ; http://n2t.net/addgene:35628 ; RRID:Addgene_35628) -
For your References section:
Comparison of the monoamine transporters from human and mouse in their sensitivities to psychostimulant drugs. Han DD, Gu HH. BMC Pharmacol. 2006 Mar 3;6:6. 10.1186/1471-2210-6-6 PubMed 16515684