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Addgene

pmNET
(Plasmid #35627)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35627 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBlueScript II SK(+)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NET
  • Alt name
    SLC6A2
  • Alt name
    norepinephrine transporter
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Slc6a2 (a.k.a. NE-T, NET, Slc6a5)
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site none (unknown if destroyed)
  • 3′ cloning site none (unknown if destroyed)
  • 5′ sequencing primer T7 fwd
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmNET was a gift from Howard Gu (Addgene plasmid # 35627 ; http://n2t.net/addgene:35627 ; RRID:Addgene_35627)

  • For your References section:

    Comparison of the monoamine transporters from human and mouse in their sensitivities to psychostimulant drugs. Han DD, Gu HH. BMC Pharmacol. 2006 Mar 3;6:6. 10.1186/1471-2210-6-6 PubMed 16515684
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