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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 35626 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneNA
- Backbone size (bp) 6290
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Vector typeMammalian Expression ; Reporter
- Promoter CMV
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Selectable markersNeomycin (select with G418)
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Tag
/ Fusion Protein
- GFPns (N terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Meant to be used in combination with pAAV-Ptet-RFP-shR-rtTA (Addgene #35625) to test the efficacy of shRNAs.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGFPns-reporter was a gift from Howard Gu (Addgene plasmid # 35626 ; http://n2t.net/addgene:35626 ; RRID:Addgene_35626) -
For your References section:
Fluorescence-based evaluation of shRNA efficacy. Naughton BJ, Han DD, Gu HH. Anal Biochem. 2011 Oct 1;417(1):162-4. Epub 2011 Jun 13. 10.1016/j.ab.2011.06.008 PubMed 21726522
Map uploaded by the depositor.
