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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 35624 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-N3
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Backbone manufacturerContech
- Backbone size w/o insert (bp) 4700
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name5ht6
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Alt name5htr6
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Alt name5-HT6
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SpeciesM. musculus (mouse)
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Entrez GeneHtr6 (a.k.a. 5-HT6)
- Promoter CMV
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Tag
/ Fusion Protein
- EGFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer EGFP-N (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFPN3-5ht6 was a gift from Kirk Mykytyn (Addgene plasmid # 35624 ; http://n2t.net/addgene:35624 ; RRID:Addgene_35624) -
For your References section:
Identification of ciliary localization sequences within the third intracellular loop of G protein-coupled receptors. Berbari NF, Johnson AD, Lewis JS, Askwith CC, Mykytyn K. Mol Biol Cell. 2008 Apr;19(4):1540-7. Epub 2008 Feb 6. 10.1091/mbc.e07-09-0942 PubMed 18256283