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Addgene

pEGFPN3-Sstr3
(Plasmid #35623)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35623 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N3
  • Backbone manufacturer
    Contech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sstr3
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Sstr3 (a.k.a. Smstr-3, Smstr3, sst3)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer EGFP-N
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFPN3-Sstr3 was a gift from Kirk Mykytyn (Addgene plasmid # 35623 ; http://n2t.net/addgene:35623 ; RRID:Addgene_35623)

  • For your References section:

    Identification of ciliary localization sequences within the third intracellular loop of G protein-coupled receptors. Berbari NF, Johnson AD, Lewis JS, Askwith CC, Mykytyn K. Mol Biol Cell. 2008 Apr;19(4):1540-7. Epub 2008 Feb 6. 10.1091/mbc.e07-09-0942 PubMed 18256283
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