pPTD-DRBD
(Plasmid #35622)

• Depositing Lab: Steven Dowdy
• Publication: Eguchi et al Nat Biotechnol. 2009 Jun;27(6):567-71. Epub 2009 May 17.

Backbone

• Vector backbone: pTAT2.2
• Backbone manufacturer: Addgene plasmid #35621
• Backbone size w/o insert (bp): 5500
• Modifications to backbone: T7 tag through NotI site of MCS are replaced with TAT-HA-TAT-DRBD.
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PKR DRBD-1
• Alt name: double-stranded RNA-binding domain
• Insert Size (bp): 210
• Promoter: T7
• Tags / Fusion Proteins:
  • TAT (N terminal on backbone)
  • TAT (N terminal on backbone)
  • HA (N terminal on backbone)
  • TAT (N terminal on backbone)
  • 6xHIS (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: None (unknown if destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7 fwd

Resource Information

• Supplemental Documents:
  • pPTD-DRBD insert sequence

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry