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Addgene

pTAT2.1
(Plasmid #35620)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35620 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b
  • Backbone manufacturer
    EMD biosciences
  • Backbone size (bp) 5368
  • Modifications to backbone
    TAT domain (RKKRRQRRR) added between 6xHIS and MCS (BamHI-EcoRI-SacI-SalI-HindIII-NotI-XhoI). See attached map for cloning information.
  • Vector type
    Bacterial Expression
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHIS (N terminal on backbone)
    • TAT (N terminal on backbone)
    • 6xHIS (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTAT2.1 was a gift from Steven Dowdy (Addgene plasmid # 35620 ; http://n2t.net/addgene:35620 ; RRID:Addgene_35620)

  • For your References section:

    Transducible TAT-HA fusogenic peptide enhances escape of TAT-fusion proteins after lipid raft macropinocytosis. Wadia JS, Stan RV, Dowdy SF. Nat Med. 2004 Mar;10(3):310-5. Epub 2004 Feb 8. 10.1038/nm996 PubMed 14770178
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