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Addgene

pTAT-CRE
(Plasmid #35619)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35619 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28
  • Backbone manufacturer
    EMD biosciences
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CRE
  • Species
    Bacteriophage P1
  • Entrez Gene
    cre (a.k.a. P1_gp003)
  • Promoter T7
  • Tags / Fusion Proteins
    • TAT (N terminal on backbone)
    • T7 tag (N terminal on backbone)
    • 6xHIS (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7 fwd
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTAT-CRE was a gift from Steven Dowdy (Addgene plasmid # 35619 ; http://n2t.net/addgene:35619 ; RRID:Addgene_35619)

  • For your References section:

    Transducible TAT-HA fusogenic peptide enhances escape of TAT-fusion proteins after lipid raft macropinocytosis. Wadia JS, Stan RV, Dowdy SF. Nat Med. 2004 Mar;10(3):310-5. Epub 2004 Feb 8. 10.1038/nm996 PubMed 14770178
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