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Addgene

pTAT-HA
(Plasmid #35612)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35612 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pREST-A
  • Backbone manufacturer
    Invitrogen
  • Backbone size (bp) 3000
  • Modifications to backbone
    TAT domain (YGRKKRRQRRR) with 5' 6xHIS and 3' HA followed by MCS consisting of NcoI-KpnI-AgeI-XhoI-SphI-EcoRI-BstBI. See attached map for full cloning information.
  • Vector type
    Bacterial Expression
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHIS (N terminal on backbone)
    • TAT (N terminal on backbone)
    • HA (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTAT-HA was a gift from Steven Dowdy (Addgene plasmid # 35612 ; http://n2t.net/addgene:35612 ; RRID:Addgene_35612)

  • For your References section:

    Transduction of full-length TAT fusion proteins into mammalian cells: TAT-p27Kip1 induces cell migration. Nagahara H, Vocero-Akbani AM, Snyder EL, Ho A, Latham DG, Lissy NA, Becker-Hapak M, Ezhevsky SA, Dowdy SF. Nat Med. 1998 Dec;4(12):1449-52. 10.1038/4042 PubMed 9846587
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