pcDNA-zeo: alpha6mGFP
(Plasmid #35611)

• Depositing Lab: Henry Lester
• Publication: Xiao et al Biochem Pharmacol. 2011 Oct 15;82(8):852-61. Epub 2011 May 17.

Backbone

• Vector backbone: pcDNA3.1-zeo
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5015
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: nAChr alpha6mGFP
• Alt name: alpha6mGFP
• Alt name: Chrna6mGFP
• Species: M. musculus (mouse)
• Insert Size (bp): 2219
• Mutation: mGFP inserted after A405 with A-G-A linker on either side
• GenBank ID: NM_021369.2
• Entrez Gene: Chrna6 (a.k.a. Acra6, Nica6)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH-Rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: synthesized from alpha6 I.M.A.G.E. cDNA ID no. 4501558

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry