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pcDNA-zeo: alpha6mGFP
(Plasmid #35611)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35611 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1-zeo
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5015
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    nAChr alpha6mEGFP
  • Alt name
    alpha6mEGFP
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2219
  • GenBank ID
    NM_021369.2
  • Entrez Gene
    Chrna6 (a.k.a. Acra6, Nica6)
  • Promoter CMV
  • Tag / Fusion Protein
    • mEGFP inserted after A405 with AGA linker on either side

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH-Rev
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    synthesized from alpha6 I.M.A.G.E. cDNA ID no. 4501558

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA-zeo: alpha6mGFP was a gift from Henry Lester (Addgene plasmid # 35611 ; http://n2t.net/addgene:35611 ; RRID:Addgene_35611)

  • For your References section:

    Characterizing functional alpha6beta2 nicotinic acetylcholine receptors in vitro: mutant beta2 subunits improve membrane expression, and fluorescent proteins reveal responsive cells. Xiao C, Srinivasan R, Drenan RM, Mackey ED, McIntosh JM, Lester HA. Biochem Pharmacol. 2011 Oct 15;82(8):852-61. Epub 2011 May 17. 10.1016/j.bcp.2011.05.005 PubMed 21609715
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