pcDNA3-FRB-LplA(W37V)
(Plasmid
#35608)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 35608 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
- Backbone size w/o insert (bp) 6000
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFRB-LplA(W37V)
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Insert Size (bp)1371
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MutationLplA has Trp37 to Val mutation
- Promoter CMV
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Tags
/ Fusion Proteins
- His Tag (N terminal on backbone)
- HA (N terminal on insert)
- FLAG (internal fusion)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3-FRB-LplA(W37V) was a gift from Alice Ting (Addgene plasmid # 35608 ; http://n2t.net/addgene:35608 ; RRID:Addgene_35608) -
For your References section:
Imaging Protein-Protein Interactions inside Living Cells via Interaction-Dependent Fluorophore Ligation. Slavoff SA, Liu DS, Cohen JD, Ting AY. J Am Chem Soc. 2011 Dec 14;133(49):19769-76. Epub 2011 Nov 18. 10.1021/ja206435e PubMed 22098454
