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pUASTattB_NSnoFbox-vhhGFP4
(Plasmid #35578)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35578 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUASattB
  • Backbone size w/o insert (bp) 8500
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NSnoFbox-vhhGFP4
  • Alt name
    slmb
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    795
  • Mutation
    F box domain of slmb deleted.
  • GenBank ID
    AY118898
  • Entrez Gene
    slmb (a.k.a. Dmel_CG3412, BcDNA:GM02031, CG3412, Dmel\CG3412, FBXW1, MENE (3R)-B, MENE(3R)-B, SLIMB, SLMB, Slimb, Slimb/Beta-TRCP1, Slimb/beta-TRCP, Slimb/beta-TrCP, Slimb/betaTrCP, Slmb, beta-TrCP, betaTrCP, crd, ica, l(3)00295, shv, slimb, wel)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer caactactgaaatctgcc
  • 3′ sequencing primer ctctgtaggtagtttgtcc
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUASTattB_NSnoFbox-vhhGFP4 was a gift from Markus Affolter (Addgene plasmid # 35578 ; http://n2t.net/addgene:35578 ; RRID:Addgene_35578)

  • For your References section:

    Fluorescent fusion protein knockout mediated by anti-GFP nanobody. Caussinus E, Kanca O, Affolter M. Nat Struct Mol Biol. 2011 Dec 11;19(1):117-21. doi: 10.1038/nsmb.2180. 10.1038/nsmb.2180 PubMed 22157958
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