MS2-HB
(Plasmid #35573)

• Depositing Lab: Marian Waterman
• Publication: Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110.007385. Epub 2011 Feb 1.

Backbone

• Vector backbone: pQCXIP
• Backbone manufacturer: Qiagen
• Backbone size w/o insert (bp): 7480
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: MS2 Coat Protein
• Alt name: N55K
• Species: Bacteriophage
• Insert Size (bp): 777
• Mutation: Insert is a tail-to-head tandem placement of two MS2 coat protein open reading frames. Both MS2 contains a N55K mutation that increases protein affinity to MS2 stem loop
• Entrez Gene: cp (a.k.a. MS2g2)
• Promoter: CMV
• Tag / Fusion Protein:
  • HTBH (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Not1 (not destroyed)
• 3′ cloning site: Pac1 (not destroyed)
• 5′ sequencing primer: LNCX
• 3′ sequencing primer: IRES-R

Resource Information

• Supplemental Documents:
  • Protein sequence
• A portion of this plasmid was derived from a plasmid made by: MS2 coat protein sequence was amplified from pCT119-N55K (gift from David Peabody, University of New Mexico).
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry