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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 35572 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRSTF
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Backbone manufacturerJang et al. 2004
- Backbone size (bp) 4660
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Modifications to backboneRenilla Luciferase open reading frame removed. 4 Ms2 stem loop sequences cloned downstream of Firefly Luciferase.
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Vector typeMammalian Expression, Luciferase
- Promoter SV40
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Tag
/ Fusion Protein
- Ms2 Stem Loop (C terminal on backbone)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Tagged-Cap was a gift from Marian Waterman (Addgene plasmid # 35572 ; http://n2t.net/addgene:35572 ; RRID:Addgene_35572) -
For your References section:
Quantitative profiling of in vivo-assembled RNA-protein complexes using a novel integrated proteomic approach. Tsai BP, Wang X, Huang L, Waterman ML. Mol Cell Proteomics. 2011 Apr;10(4):M110.007385. Epub 2011 Feb 1. 10.1074/mcp.M110.007385 PubMed 21285413
Map uploaded by the depositor.
