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pcDNA Flag-RARalpha (T181A,S445A,S461A)
(Plasmid #35556)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35556 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA 6
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RARalpha
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1386
  • Mutation
    T181A, S445A, S461A
  • Entrez Gene
    RARA (a.k.a. NR1B1, RAR, RARalpha)
  • Tags / Fusion Proteins
    • His (N terminal on backbone)
    • Flag (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA Flag-RARalpha (T181A,S445A,S461A) was a gift from Jonathan Kurie & Harish Srinivas (Addgene plasmid # 35556 ; http://n2t.net/addgene:35556 ; RRID:Addgene_35556)

  • For your References section:

    c-Jun N-terminal kinase contributes to aberrant retinoid signaling in lung cancer cells by phosphorylating and inducing proteasomal degradation of retinoic acid receptor alpha. Srinivas H, Juroske DM, Kalyankrishna S, Cody DD, Price RE, Xu XC, Narayanan R, Weigel NL, Kurie JM. Mol Cell Biol. 2005 Feb;25(3):1054-69. 10.1128/MCB.25.3.1054-1069.2005 PubMed 15657432
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