p4xSTAT6-Luc2P
(Plasmid #35554)

• Depositing Lab: Axel Nohturfft
• Publication: pCMV-STAT6-IRES-Neo (unpublished)

Backbone

• Vector backbone: pGL4
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4267
• Modifications to backbone: Plasmid pGL4.32[luc2P/NF-κB-RE/Hygro] (Promega) was modified by removing the 1782-bp XbaI fragment and by replacing the NF-κB promoter.
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: 4xStat6 promoter
• Species: H. sapiens (human), Synthetic
• Insert Size (bp): 156
• Promoter: 4xStat6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: BglII (destroyed during cloning)
• 5′ sequencing primer: RVprimer3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Note: The design of the promoter insert is based on a reporter plasmid, TPU474, previously described by Mikita et al., 1996 [PMID 8816495]
• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid was generated by replacing the HIndIII(blunt)-AdeI fragment of p2xSTAT6-Luc2P (Addgene plasmid 35486) with the KpnI(blunt)-AdeI fragment of p2xSTAT6-Luc2P. The promoter insert consists of four tandem copies of STAT6/cEBP binding sites, a composite element from the human germ line ε promoter.
Lab ID for this plasmid: pANG193.