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Addgene

Tet-O-FUW-FoxG1
(Plasmid #35547)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35547 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    TetO-FUW
  • Backbone size w/o insert (bp) 8393
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FoxG1
  • Alt name
    Bf1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1452
  • Entrez Gene
    Foxg1 (a.k.a. 2900064B05Rik, BF-1, Bf1, Hfh9, Hfhbf1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer LNCX
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tet-O-FUW-FoxG1 was a gift from Marius Wernig (Addgene plasmid # 35547 ; http://n2t.net/addgene:35547 ; RRID:Addgene_35547)

  • For your References section:

    Direct conversion of mouse fibroblasts to self-renewing, tripotent neural precursor cells. Lujan E, Chanda S, Ahlenius H, Sudhof TC, Wernig M. Proc Natl Acad Sci U S A. 2012 Feb 14;109(7):2527-32. Epub 2012 Jan 30. 10.1073/pnas.1121003109 PubMed 22308465
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