pCI-neo-RL-ZEB1 200bmutx5
(Plasmid #35537)

• Depositing Lab: Greg Goodall
• Publication: Gregory et al Nat Cell Biol. 2008 May;10(5):593-601. Epub 2008 Mar 30.

Backbone

• Vector backbone: pCI-neo-RL
• Backbone manufacturer: Witold Filipowicz
• Backbone size w/o insert (bp): 6380
• Total vector size (bp): 8144
• Modifications to backbone: RL was inserted into pCI-neo (Promega) to form the plasmid pcI-neo-RL by Witold Filipowicz
• Vector type: Mammalian Expression, Luciferase
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: 3'UTR of ZEB1
• Alt name: BZP; TCF8; AREB6; FECD6; NIL2A; PPCD3; ZFHEP; ZFHX1A; DELTAEF1
• Species: H. sapiens (human), S. cerevisiae (budding yeast)
• Insert Size (bp): 1764
• Mutation: 5 mutations in miR200b seed regions of 3'UTR insert
• GenBank ID: 6935
• Entrez Gene: ZEB1 (a.k.a. AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP, ZFHX1A)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (destroyed during cloning)
• 3′ cloning site: XbaI (destroyed during cloning)
• 5′ sequencing primer: unknown
• 3′ sequencing primer: unknown

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Mutations in the miR-200b seed regions of the ZEB1 3'UTR were generated using the QuikChange Multi site-directed mutagenesis kit.
The ZEB1 3'UTR was cloned into the plasmid pCIneo-RL which was obtained from Witold Filiowicz.