Skip to main content
Addgene

pLenti 4.1 Ex miR200c-141
(Plasmid #35534)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35534 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pLenti 4.1Ex
  • Backbone manufacturer
    Scott Hammond
  • Backbone size w/o insert (bp) 8749
  • Total vector size (bp) 9439
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    miR200c-141 genomic cluster
  • Alt name
    MIRN200C
  • Alt name
    MIRN141
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    690
  • GenBank ID
    406985 406933
  • Entrez Gene
    MIR200C (a.k.a. MIRN200C, mir-200c)
  • Entrez Gene
    MIR141 (a.k.a. MIRN141, mir-141)
  • Promoter CMV
  • Tag / Fusion Protein
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site AvrII (destroyed during cloning)
  • 5′ sequencing primer Unknown
  • 3′ sequencing primer unknown
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti 4.1 Ex miR200c-141 was a gift from Greg Goodall (Addgene plasmid # 35534 ; http://n2t.net/addgene:35534 ; RRID:Addgene_35534)

  • For your References section:

    The miR-200 family and miR-205 regulate epithelial to mesenchymal transition by targeting ZEB1 and SIP1. Gregory PA, Bert AG, Paterson EL, Barry SC, Tsykin A, Farshid G, Vadas MA, Khew-Goodall Y, Goodall GJ. Nat Cell Biol. 2008 May;10(5):593-601. Epub 2008 Mar 30. 10.1038/ncb1722 PubMed 18376396
Related items:
Commonly requested with: