pQCXIP-Rx3(A8)-mEGFP
(Plasmid
#35532)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 35532 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepQCXIX
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Backbone manufacturerclontech
- Backbone size w/o insert (bp) 6300
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Modifications to backbonepuro marker at MCS2
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRx3A8mEGFP
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SpeciesSynthetic
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Insert Size (bp)984
- Promoter CMV
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Tag
/ Fusion Protein
- mEGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Not1 (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pQCXIP-Rx3(A8)-mEGFP was a gift from Robert Cohen (Addgene plasmid # 35532 ; http://n2t.net/addgene:35532 ; RRID:Addgene_35532) -
For your References section:
Polyubiquitin-sensor proteins reveal localization and linkage-type dependence of cellular ubiquitin signaling. Sims JJ, Scavone F, Cooper EM, Kane LA, Youle RJ, Boeke JD, Cohen RE. Nat Methods. 2012 Feb 5. doi: 10.1038/nmeth.1888. 10.1038/nmeth.1888 PubMed 22306808
