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Addgene

pRSLeu2d-BISopt
(Plasmid #35524)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35524 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS425ADS-Leu2d
  • Vector type
    Yeast Expression, Synthetic Biology
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Bisabolene Synthase
  • Species
    Abies grandis
  • Promoter P GAL 1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xba1 (unknown if destroyed)
  • 3′ cloning site Xho1 (unknown if destroyed)
  • 5′ sequencing primer agttctagaatccggggt
  • 3′ sequencing primer CATGTAATTAGTTATG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSLeu2d-BISopt was a gift from Taek Soon Lee (Addgene plasmid # 35524 ; http://n2t.net/addgene:35524 ; RRID:Addgene_35524)

  • For your References section:

    Identification and microbial production of a terpene-based advanced biofuel. Peralta-Yahya PP, Ouellet M, Chan R, Mukhopadhyay A, Keasling JD, Lee TS. Nat Commun. 2011 Sep 27;2:483. doi: 10.1038/ncomms1494. 10.1038/ncomms1494 PubMed 21952217
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