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Addgene

pCMV-STAT6-IRES-Neo
(Plasmid #35482)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35482 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV-SPORT6
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4000
  • Modifications to backbone
    Vector also contains an IRES-Neo insert
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    STAT6
  • Alt name
    Signal transducer and activator of transcription 6
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2544
  • GenBank ID
    BC075852.1
  • Entrez Gene
    STAT6 (a.k.a. D12S1644, HIES6, IL-4-STAT, STAT6B, STAT6C)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-forward
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The precursor to this plasmid was an IMAGE clone that was purchased from Source Bioscience, UK.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To generate pCMV-STAT6-IRES-Neo, the NotI-XbaI fragment of pCMV-Stat6 (IMAGE clone info: clone="MGC:90177 IMAGE:5530399") was replaced with that of pCMV-Fcgamma-RIIa-IRES-neo (Addgene plasmid ID 14948).
Lab ID for pCMV-STAT6-IRES-Neo: pANG194.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-STAT6-IRES-Neo was a gift from Axel Nohturfft (Addgene plasmid # 35482 ; http://n2t.net/addgene:35482 ; RRID:Addgene_35482)
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