pRSII323
(Plasmid #35463)

• Purpose: (Empty Backbone)
• Depositing Lab: Steven Haase
• Publication: Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epub 2012 May 1.

Backbone

• Vector backbone: pRS32x
• Backbone manufacturer: Stratagene
• Backbone size (bp): 5797
• Modifications to backbone: 2 micron origin is from YEplac195, which has had its XbaI site removed.
• Vector type: Yeast 2 micron vector
• Promoter: None
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: M13 Forward, T7
• 3′ sequencing primer: M13 Reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Daniel J Lew, Duke University Medical Center Chandra Tucker, Duke University (now at University of Colorado, Denver)
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

HIS3 has been modified to remove HindIII, KpnI, and PstI sites without altering the amino acid sequence of the His3 protein.