pRSII422
(Plasmid #35462)

• Purpose: (Empty Backbone)
• Depositing Lab: Steven Haase
• Publication: Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epub 2012 May 1.

Backbone

• Vector backbone: pRS42x
• Backbone manufacturer: Stratagene
• Backbone size (bp): 6865
• Modifications to backbone: Undocumented insertion in pRS412 was eliminated in the process of constructing pRSII422. 2 micron replication origin is from YEplac195 and has had its XbaI site removed.
• Vector type: Yeast 2 micron vector
• Promoter: None
• Selectable markers: ADE2

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: M13 Forward, T7
• 3′ sequencing primer: M13 Reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Chandra Tucker, Duke University (now at University of Colorado, Denver)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

ADE2 has been modified to remove HindIII and XbaI sites without altering the amino acid sequence of the Ade2 protein.