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Addgene

pRSII319
(Plasmid #35459)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 35459 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS31x
  • Backbone manufacturer
    Stratagene
  • Backbone size (bp) 5539
  • Vector type
    Yeast centromere vector
  • Promoter None
  • Selectable markers
    HIS2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer M13 Forward, T7
  • 3′ sequencing primer M13 Reverse
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Daniel J. Lew, Duke University Medical Center
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HIS2 has been modified to remove BamHI and XhoI sites without altering the amino acid sequence of the His2 protein.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRSII319 was a gift from Steven Haase (Addgene plasmid # 35459 ; http://n2t.net/addgene:35459 ; RRID:Addgene_35459)

  • For your References section:

    New and Redesigned pRS Plasmid Shuttle Vectors for Genetic Manipulation of Saccharomycescerevisiae. Chee MK, Haase SB. G3 (Bethesda). 2012 May;2(5):515-26. Epub 2012 May 1. 10.1534/g3.111.001917 PubMed 22670222
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