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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 35449 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepRS31x
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Backbone manufacturerStratagene
- Backbone size (bp) 4965
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Modifications to backboneThe opposite orientation of CEN (centromere) sequence in original pRS313 compared with the other pRS CEN plasmids has been reversed in pRSII313.
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Vector typeYeast centromere vector
- Promoter None
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13 Forward, T7
- 3′ sequencing primer M13 Reverse (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDaniel J. Lew, Duke University Medical Center
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
HIS3 has been modified to remove HindIII, KpnI, and PstI sites without altering the amino acid sequence of the His3 protein.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRSII313 was a gift from Steven Haase (Addgene plasmid # 35449 ; http://n2t.net/addgene:35449 ; RRID:Addgene_35449) -
For your References section:
New and Redesigned pRS Plasmid Shuttle Vectors for Genetic Manipulation of Saccharomycescerevisiae. Chee MK, Haase SB. G3 (Bethesda). 2012 May;2(5):515-26. Epub 2012 May 1. 10.1534/g3.111.001917 PubMed 22670222
