pRSII408
(Plasmid #35444)

• Purpose: (Empty Backbone)
• Depositing Lab: Steven Haase
• Publication: Chee et al G3 (Bethesda). 2012 May;2(5):515-26. Epub 2012 May 1.

Backbone

• Vector backbone: pRS40x
• Backbone manufacturer: Stratagene
• Backbone size (bp): 4520
• Vector type: Yeast integrating vector
• Promoter: None
• Selectable markers: ADE1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: M13 Forward, T7
• 3′ sequencing primer: M13 Reverse

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Chandra Tucker, Duke University (now at University of Colorado, Denver) and M. Henar Valdivieso, Scripps Institutue (now at Institute of Biochemical Microbiology, Spain).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

ADE1 has been modified to remove BamHI, EcoRI, SalI and XbaI sites without altering the amino acid sequence of the Ade1 protein.

Note that there may be some small discrepancies between Addgene's quality control sequence and the the assembled from the depositor. These discrepancies are not in critical regions.