pTH-MYCN
(Plasmid #35416)

• Depositing Lab: William A. Weiss
• Publication: Weiss et al EMBO J. 1997 Jun 2;16(11):2985-95.

Backbone

• Vector backbone: pGEM7Zf(+)
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 2997
• Modifications to backbone: TH promoter and Beta-Globin Intron inserted upstream of MYCN cDNA
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10/P3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: v-myc myelocytomatosis viral related oncogene, neuroblastoma derived (avian)
• Alt name: MYCN
• Species: H. sapiens (human)
• Insert Size (bp): 2600
• GenBank ID: NM_005378
• Entrez Gene: MYCN (a.k.a. FGLDS1, MODED, MPAPA, MYCNsORF, MYCNsPEP, N-myc, NMYC, ODED, bHLHe37)
• Promoter: Tryosine Hydroxlyase (TH)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: SP6

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry