pEGFP-RNF17L
(Plasmid #35413)

• Depositing Lab: Jeremy Wang
• Publication: Pan et al Development. 2005 Sep;132(18):4029-39. Epub 2005 Aug 10.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4731
• Total vector size (bp): 10000
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Rnf17l
• Alt name: Rnf17
• Alt name: Rnf17 long transcript
• Alt name: RING finger protein 17 long transcript
• Species: M. musculus (mouse)
• Insert Size (bp): 5148
• GenBank ID: AY854011.1 AAX51691.1
• Entrez Gene: Rnf17 (a.k.a. MMIP-2, Mmip2, TDRD4)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: SmaI (unknown if destroyed)
• 5′ sequencing primer: EGFP-C; CMV-F
• 3′ sequencing primer: SV40pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Rnf17 cDNA fragments amplified and subcloned from bulk mouse testis Marathon-Ready cDNAs (BD Biosciences).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Based on previously reported Rnf17 cDNA sequences, Rnf17-specific primers for 3'RACE were designed to obtain full-length cDNA
sequences for Rnf17s and Rnf17l (Wang et al., 2001; Yin et al., 1999). By using 3'RACE (rapid amplification of cDNA ends), the depositors amplified, subcloned and sequenced Rnf17 cDNA fragments from bulk mouse testis Marathon-Ready cDNAs (BD Biosciences). The composite cDNA sequences were deposited in GenBank under the following accession numbers: Rnf17s, AY854010; Rnf17l, AY854011.

Rnf17 long transcript cDNA was cloned in frame with EGFP into pEGFP-C1 with HindIII/SmaI. (Genbank: AY854011)